The Relative Centrifugal Force (RCF) is the amount of acceleration to be applied to the sample in the centrifuge. It depends on the Rotation Speed and the Radius of the rotor, and is relative to the force of Earth’s gravity (g-Force).
Unfortunately, many protocols and instructions only list the RPMs as a parameter for correct process setup. This is OK as long as you use only one type of rotor/centrifuge, but as soon as you try to use your process disposable on a different rotor/centrifuge, the original RPM values might be wrong if the rotor diameter differs.
Therefore, you have to convert the Rotation Speed (RPM) into Relative Centrifugation Force (RCF) and vice versa. For this purpose you will need the Rotor Radius of your centrifuge. The radius is measured from the axis of rotation to the inner bottom of the swing-out cup.
The following tool allows to calculate RCF and RPM values. The Rotor Radius fields below are pre-set with the Hanil Fleta-40P rotor radius value of 172.6mm (as mentioned in the instructions).
Relative Centrifugal Force (RCF) or g-Force:
Revolutions per Minute (RPM) or Rotation Speed: